Potential of Uwi (Dioscorea alata L.) as Antiosteoporosis in Histopathological Appearance of Rat Bone (Rattus novergicus)

Ismedsyah; Lavinur; Melva  Simatupang

doi:10.54783/endlessjournal.v5i2.70

Authors

Ismedsyah Poltekkes Kemenkes Medan, Indonesia
Lavinur Poltekkes Kemenkes Medan, Indonesia
Melva Simatupang Poltekkes Kemenkes Medan, Indonesia

DOI:

https://doi.org/10.54783/endlessjournal.v5i2.70

Abstract

Estrogen deficiency can be overcome by hormone replacement therapy, but it causes various unwanted effects in the long term. Phytoestrogens meet the criteria for having estrogen-like activity. Sweet potato root (Dioscorea alata L.) as a phytoestrogen was used in this study to anticipate bone loss (osteoporosis) in postmenopausal women. This study aims to determine the potential of uwi in bone density in rats by looking at the decrease in the number of osteoclasts. Laboratory experimental research method with a research design using RAL (Rak Complete) with five treatments and six replications. The results showed that the average number of osteoclasts from the negative control group was 4.67; the positive control of 2.83; by offering uwi extract at a dose of 600 milligrams/200 grams. Rat BB 2.16; presented Uwi extract dose of 800 milligrams/200 grams of rat B.B. as much as 2.83; presented Uwi extract at a dose of 1000 milligrams/200 grams Rat BB 1.0; and 2.5 . false group. This study concludes that Uwi is likely to maintain bone density in rats with osteoporosis by decreasing the number of osteoclasts.

References

Abementhy, K., Hillard, A., McFall, P., Holloway, D., & Robinson, J. (2015). Menopause: lifestyle and therapeutic approaches. Royal College of Nursing.

Acadia, C. M. (2003). Fitoestrogen untuk Wanita menopause. Retrieved from: http://www.situs.kesrepro.info/aging/jul/2003/ag01.html

Anonim. (2010). Data Osteoporosis Pusdatin. Jakarta. Kementerian Kesehatan Republik Indonesia.

Aprilia, D. A. (2010). Terapi Suluh Hormon Pada Osteoporosis. Universitas Sumatera Utara.

Bayan, L., Koulivand, P. H., & Gorji, A. (2014). Garlic: A Review of Potential Therapeutic Effects. Avicenna Journal of Phytomedicine, 4(1), 1-14 .

Baziad, A. (2003). Menopause dan Andropause. Jakarta: Yayasan Bina Pustaka.

Beg, M., Akhtar, N., Alam, M. F., Rizvi, I., Ahmad, J., & Gupta, A. (2014). Vitamin D status and serum osteocalcin levels in post-menopausal osteoporosis: Effect of bisphosphonate therapy. JIACM, 15(3-4), 172-6.

Chaiyakunapruk, N., Laowakul, A., Karnchanarat, S., Pikulthong, N., & Ongphiphadhanakul, B. (2006). Community pharmacy–based implementation and evaluation of an osteoporosis self-assessment tool for Asians. Journal of the American Pharmacists Association, 46(3), 391-396.

Chaovisitsaree, S., Namwongprom, S. A., Morakote, N., Suntornlimsiri, N., & Piyamongkol, W. (2007). Comparison of osteoporosis self assessment tool for Asian (OSTA) and standard assessment in Menopause Clinic, Chiang Mai. Journal-Medical Association of Thailand, 90(3), 420.

Clarke, B. L., & Khosla, S. (2010). Physiology of bone loss. Radiologic Clinics, 48(3), 483-495.

Cook, R. B., Collins, D., Tucker, J., & Zioupos, P. (2005). Comparison of questionnaire and quantitative ultrasound techniques as screening tools for DXA. Osteoporosis international, 16(12), 1565-1575.

Febrina, D., & Lasmini, P. S. (2006). Gambaran Densitometry Tulang Belakang dan Femur di IDT RSUP Dr M Djamil Padang. Universitas Andalas.

Glover, A., & Assinder, S. J. (2006). Acute exposure of adult male rats to dietary phytoestrogens reduces fecundity and alters epididymal steroid hormone receptor expression. Journal of endocrinology, 189(3), 565-573.

Harahap, E. E. S. (2014). Hubungan Kadar Estradiol Serum Dengan Densitas Tulang pada Wanita Menopause. Universitas Sumatera Utara.

Jefferson, W. N., Padilla-Banks, E., Clark, G., & Newbold, R. R. (2002). Assessing estrogenic activity of phytochemicals using transcriptional activation and immature mouse uterotrophic responses. Journal of Chromatography B, 777(1-2), 179-189.

Klibanski, A., Adams-Campbell, L., Bassford, T., Blair, S. N., Boden, S. D., Dickersin, K., ... & Russell, W. E. (2001). Osteoporosis prevention, diagnosis, and therapy. Journal of the American Medical Association, 285(6), 785-795.

Lawrence, D. R., & Bacharach, A. L. (1964). Evaluation of Drug Activities: Pharmacometrics. London: Academic Press.

Prawirohardjo, S., & Badarudeen, A. (2015). Osteoporosis. Retrieved from: http://www.slideshare.net/AseemBadarudeen/osteoporosis-ppt-49486060

Raisz, L. G. (2005). Pathogenesis of osteoporosis: concepts, conflicts, and prospects. The Journal of clinical investigation, 115(12), 3318-3325.

Tao, B., Liu, J. M., Li, X. Y., Wang, J. G., Wang, W. Q., & Ning, G. (2008). An assessment of the use of quantitative ultrasound and the Osteoporosis Self-Assessment Tool for Asians in determining the risk of nonvertebral fracture in postmenopausal Chinese women. Journal of Bone and Mineral Metabolism, 26(1), 60-65.

Thomas, A. N. S. (2012). Tanaman Obat Tradisional 1. Yogyakarta: Kanisius.

Thompson, L. U., Boucher, B. A., Liu, Z., Cotterchio, M., & Kreiger, N. (2006). Phytoestrogen content of foods consumed in Canada, including isoflavones, lignans, and coumestan. Nutrition and cancer, 54(2), 184-201.

van Steenis, C. G. G. J. (2008). Flora. Jakarta: Pradnya Paramita.